Equipment for PCR lab

Equipping FOR PCR laboratory in Ukraine

Polymerase chain reaction (PCR) is an experimental method of molecular biology that allows achieving a significant increase in small concentrations of certain fragments of nucleic acid (DNA) in a biological material (sample).

In addition to DNA amplification, PCR allows many other manipulations with nucleic acids (introduction of mutations, splicing of DNA fragments) and is widely used in biological and medical practice, for example for the diagnosis of diseases (hereditary, infectious), for paternity, for gene cloning, isolation new genes.

Polymerase chain reaction is the most modern and relevant method in clinical and scientific research, oncology, criminology and veterinary medicine, because PCRs have the following advantages:

• PCR is a direct method and achieves the highest possible sensitivity: from several copies of the genetic target in the sample
• The specificity of the method is 100%
• Different types of material are suitable for PCR analysis, including histological preparations
• The amount of the test material, as a rule, is several tens of microliters
• It is possible to determine the number of copies of the genetic target of the pathogen in the sample, to control viremia or bacteremia during treatment
• Ease of execution and the possibility of full automation
• The possibility of obtaining results within a few hours after the collection of the material
• It is possible to identify several pathogens in one PCR test, which leads to a reduction in testing time, reduces costs, and leads to a reduction in the number of manipulations, which leads to a reduction in the probability of errors in the PCR study

Diameb Trade offers a unique wide range of AmpliSens reagent kits for all stages of PCR diagnostics of human infectious diseases, veterinary medicine, as well as reagent kits for detecting the species belonging to animal tissues and to identify genetically modified ingredients in food and animal feed.

AmpliSens reagent kits were developed and produced by the Central Research Institute of Epidemiology. The nomenclature of reagent kits includes more than 600 items, of which a significant part is unique and does not have world analogues.

Stages of PCR analysis

The PCR assay is divided into the following laboratory steps:

• Extraction
• Manual
• Express
• Classical
• Sorbent method
• Precipitation method
87
• Automatic
• Amplification
• Detection
• FEP (end-point fluorescence) using ALA 1/4 from Biosan (Latvia), Rotor Gene Q from Qiagen (Germany)
• FRT (real-time fluorescence) using Rotor Gene Q from Qiagen (Germany)